tubulin polymerization assay kit (Cytoskeleton Inc)
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tubulin polymerization assay kit
Tubulin Polymerization Assay Kit, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 714 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tubulin+polymerization+assay+kit/Tubulin+polymerization+assay+using+%3E99%25+pure+tubulin%2C+OD+based+-+Porcine/pmc13620138-544-7-12
Average 96 stars, based on 714 article reviews
Tubulin Polymerization Assay Kit, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 714 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tubulin+polymerization+assay+kit/Tubulin+polymerization+assay+using+%3E99%25+pure+tubulin%2C+OD+based+-+Porcine/pmc13620138-544-7-12
Average 96 stars, based on 714 article reviews
tubulin polymerization assay kit - by Bioz Stars,
2026-10
96/100 stars
Images
1) Product Images from "CPT1A-mediated IDO1 succinylation shapes EGFRvIII-driven resistance to tumor electric field therapy in glioblastoma"
Article Title: CPT1A-mediated IDO1 succinylation shapes EGFRvIII-driven resistance to tumor electric field therapy in glioblastoma
Journal: Nature Communications
doi: 10.1038/s41467-026-77927-w
Figure Legend Snippet: A Western blot analysis of AhR in cytoplasmic and nuclear fractions of TBD0220 and LN229-EGFRvIII cells. TEFT treatment promotes AhR nuclear translocation, B Immunofluorescence staining of AhR (red) and DAPI (blue). TEFT induces AhR nuclear accumulation, which is reversed by IDO1 knockdown or osimertinib. Scale bars, 10 μm, C ATAC-seq footprinting showing increased chromatin accessibility at the AhR binding motif in EGFRvIII cells versus wild-type, D GO enrichment analysis of AhR target genes (identified by intersecting ATAC-seq and ChIP-Atlas), highlighting “Mitotic Cell Cycle” and “Microtubule Cytoskeleton” as top pathways, E Representative immunofluorescence images of mitotic spindles (α-tubulin, green) and chromosomes (DAPI, blue) in LN229 cells. TEFT causes severe spindle disruption and multipolar mitosis in wild-type cells, whereas EGFRvIII cells maintain bipolar spindles. This protection is lost upon IDO1 knockdown or osimertinib treatment, F Quantification of cells with abnormal nuclei following TEFT treatment ( n = 3 biological replicates per group), G Venn diagram intersecting RNA-seq (upregulated), ATAC-seq (accessible peaks), and ChIP-Atlas (AhR binding targets) data, identifying DCLK1 as a key downstream target, H Western blot confirming the upregulation of DCLK1 protein in EGFRvIII cells upon TEFT, I Immunofluorescence images of mitotic spindles in DCLK1 -knockdown cells. Silencing DCLK1 sensitizes EGFRvIII cells to TEFT-induced spindle defects, J Quantification of abnormal nuclear morphology in DCLK1-depleted cells ( n = 3 biological replicates per group), K Venn diagram integrating multi-omics data with microtubule/GTPase-related genes, identifying ARHGEF2 (GEF-H1) as another target, L In vitro tubulin polymerization assay in TBD0220 cells under the indicated conditions. IDO1 knockdown, ARHGEF2 knockdown, osimertinib, or epacadostat reduced the microtubule-polymerizing activity associated with EGFRvIII-mediated TEFT resistance. Data are presented as mean ± SD. The exact n values and definitions of biological replicates are specified for the relevant panels. Statistical tests were as follows: two-tailed unpaired Student’s t test for panel J ; ordinary one-way ANOVA followed by two-sided Šídák-adjusted multiple-comparisons tests for panel F . Exact P values, adjusted for multiple comparisons where applicable, are shown in the corresponding panels. Source data are provided as a Source Data file.
Techniques Used: Western Blot, Analysis, Translocation Assay, Immunofluorescence, Staining, Knockdown, Footprinting, Binding Assay, Disruption, RNA Sequencing, Morphology, Biomarker Discovery, In Vitro, Polymerization Assay, Activity Assay, Two Tailed Test
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Membrane:Article Title: In vitro toxicity of piperazine derivatives involves mitochondrial dysfunction and microtubule-related changes in neuronal cell models Article Snippet: .. The LDH Cytotoxicity Detection Kit was obtained from Roche Diagnostics, Mannheim, Germany, the TMRE Mitochondrial Membrane Potential Assay Kit from Abcam, Cambridge, UK, and the |
